Better Imaging of Living Animals and the 3Rs

Recently, I was explaining my research to another academic from a different field. I was describing how, when imaging living animals under the microscope, we strive to keep laser power levels to the minimum needed. The question I got in return was: “Is that for the animal’s sake or for the imaging?”. And of course the answer is both – in general, I find that good science and better microscopy align very well with performing more humane animal research.

This article is about how researchers are working to improve animal research by minimising numbers used, sharing animals and data and refining experimental procedures.  This is not an article on whether or not animal experimentation should or should not be done; that is a matter of personal ethics. However, should you be interested in the rules and regulations surrounding animal research in the UK, I would suggest you start with Understanding Animal Research [1] and the National Centre for the Replacement, Refinement and Reduction of Animals in Research (the NC3RS) [2].

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Let There be Light… but Not Too Much… Phototoxicity in Microscopy

Light is an Essential Aspect of Microscopy

An essential aspect to microscopy is light. Early microscopes used ambient light or used mirrors to reflect light, either from the sun or a candle, onto the sample of interest. With the inventions [1] of the electric light bulb additional, artificial light sources could be used and the light from lamps could be focussed onto samples with much greater control.

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