Imaging the developing heart: synchronized time-lapse microscopy during developmental changes

Abstract

How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to In Vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological question.

Publication
Proceedings of SPIE
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Chas Nelson
EPSRC Doctoral Prize Research Fellow

An interdisciplinary scientist with a background in quantitative microscopy and bioimage analysis.