Imaging the developing heart: synchronized time-lapse microscopy during developmental changest

Abstract

How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to in vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological question.

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Location
San Fransisco, CA USA
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Chas Nelson
LKAS Research Fellow in Data Science

An interdisciplinary scientist with a background in quantitative microscopy and bioimage analysis.